DNA and RNA extraction services is a difficult challenge and something that requires high-quality equipment, and precision. After RNA is extracted, it is important that it is checked for quality before it is used for testing, profiling or genotyping.
Once the RNA has been extracted, it can be used for next-generation sequencing, gene expression profiling, and for genotyping as well.
The procedure is one that must be followed carefully. To obtain high-quality RNA, you will need to ensure that the samples that you collect are high quality. The treatment and the handling of any sample that you collect must be methodical, and any RNA isolation must be done in the presence of the right inhibitory agents. Phenol-based compounds can help to reduce the risk of the RNA degrading, however, the two risk periods for the samples are typically prior to the isolation, and immediately after it, when it is easily possible for the RNA to be damaged.
It is important that you collect the best tissues for the samples. The tissue samples will need to be disrupted, and you must ensure that the lytic agent is in contact with the cell contents as quickly as possible, otherwise, the samples could decay. It can be hard to ensure the contact with the denaturant if the tissue or cell is something like bone or hard roots, or if there are walls or capsules – as there are in the case of gram-positive bacteria and spores.
Important information from tepnelpharmaservices – Freezing the tissues or cells in dry ice or liquid nitrogen can help to ensure that you get the best possible results. The process will give you more control over the purification conditions – however, it can be time-consuming and a challenge.
There are RNA stabilization solutions which allow you to preserve the integrity of the RNA even if you opt to delay the processing and extraction of the RNA. These solutions mean that the samples can be collected when the opportunity arises, and then the isolation can be done days or even weeks later, perhaps in batches to save time and funds. Samples can be shipped to another lab if necessary, without worrying about degradation impairing the quality of the results or the usefulness of the sample that comes out.